Luminol reagent 521-31-3: an efficient detection tool in Western blot experiments

In the fields of molecular biology and biochemistry research, Western blotting is a classic and critical technique widely used to detect the expression of specific proteins in complex samples. The introduction of luminol reagent has significantly improved this experimental technique and become an indispensable detection tool in Western blot experiments.

1, Luminol's excellent luminescent properties

The core advantage of luminol luminescent reagents lies in their excellent luminescent performance. In Western blot experiments, when the target protein binds to a specific antibody, the luminol luminescent reagent can undergo a chemical reaction under the action of catalysts such as hydrogen peroxide, producing chemiluminescence phenomenon. This luminescent process has extremely high sensitivity and can detect target proteins at very low concentrations. Even if the content of the target protein in the sample is negligible, the luminol luminescent reagent can release a strong enough light signal, allowing researchers to clearly observe protein bands. This highly sensitive detection capability enables researchers to analyze protein expression levels more accurately, providing strong support for further exploration of protein functions and regulatory mechanisms.

luminol powder

2, Fast and stable luminescent reaction

The luminescence reaction of luminol luminescent reagent is not only sensitive, but also extremely fast and stable. After adding a catalyst, luminol can quickly react with hydrogen peroxide and produce a strong luminescent signal in a short period of time. This feature greatly shortens the detection time of protein blotting experiments, allowing researchers to obtain experimental results in a shorter period of time.

3, Good compatibility

Luminol luminescent reagents have good compatibility and can be used in conjunction with various antibody detection systems. Whether using direct or indirect labeling methods, luminol luminescent reagents can demonstrate excellent detection performance. In the direct labeling method, luminol can directly bind to specific antibodies to achieve direct detection of target proteins; In the indirect labeling method, luminol binds to the secondary antibody and indirectly detects the target protein through the specific binding of the secondary antibody to the primary antibody. This broad compatibility allows luminol luminescent reagents to be flexibly applied to different experimental designs and sample types, meeting the diverse research needs of researchers.

4, Low background interference, improving detection specificity

In Western blot experiments, background interference is one of the important factors affecting the accuracy of detection results. Luminol luminescent reagents have the characteristic of low background interference, which can effectively reduce the generation of non-specific signals. Its luminescence reaction has high specificity, and it only triggers luminescence in the presence of antibodies that bind to the target protein, greatly reducing the intensity of the background signal. This enables researchers to more clearly distinguish target protein bands, improving the specificity and accuracy of detection.

Product packaging

The luminol reagent produced by Hubei Xindesheng Material Technology Co., Ltd. can reach over 99% and has a white powder like appearance. Desheng has rich R&D and production experience, and has cooperated with numerous domestic and foreign enterprises and laboratories, receiving high praise from customers! If you have any purchasing needs in the near future, please feel free to contact me at any time!