Core advantages of chromogenic substrate TOOS in adenosine deaminase assay

Adenosine deaminase (ADA) is a key enzyme in purine nucleoside metabolism, and its activity changes are closely related to pathological states such as liver cell injury, immune dysfunction, and tuberculosis infection. In clinical biochemical testing, the chromogenic substrate TOOS reagent (N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt) has become the core reagent for ADA activity determination due to its unique chemical properties and reaction mechanism, providing a highly sensitive and stable solution for disease diagnosis.

1, High sensitivity color development: precise capture of trace enzyme activity

One of the core advantages of TOOS is its excellent molar absorbance. The sulfonic and methylaniline groups in the molecular structure endow it with high electron density. Under the catalysis of peroxidase (POD), it undergoes coupling reaction with hydrogen peroxide (H ₂ O ₂) and 4-aminoantipyrine (4-AA) to form a red quinone imine compound. Its molar absorbance coefficient is much higher than that of traditional chromogenic substrates, and it can produce significant color changes at extremely low ADA concentrations.

TOOS powder

2, Strong anti-interference ability: stable performance in complex samples

Clinical serum samples often contain interfering substances such as bilirubin, hemoglobin, triglycerides, etc. TOOS effectively reduces non-specific adsorption through its hydrophilic sulfonic group and molecular steric hindrance effect. In addition, its sulfonic structure can inhibit the competitive binding of cations such as ammonium ions, ensuring high fidelity color development in complex matrices.

3, Rapid color reaction: shorten the detection cycle

The coupling reaction rate involving TOOS is significantly higher than that of traditional Trinder's reagents. The coupling reaction between TOOS and H ₂ O ₂ during the ADA catalyzed conversion of adenine nucleoside to hypoxanthine and further oxidation to H ₂ O ₂ can reach the color plateau period in a short time, shortening the detection time compared to traditional methods. This feature is particularly important in emergency liver injury diagnosis, large-scale tuberculosis screening, and other scenarios, which can significantly improve detection throughput and efficiency.

4, Highly water-soluble formula: simplified operation process

TOOS has high solubility, far exceeding traditional aniline based color reagents. Its aqueous solution is stable at room temperature and does not require organic solvents for solubilization. It can be directly prepared as a two-component working solution with reagents such as 4-AA and POD. This "ready to use" feature not only simplifies the detection process, but also reduces inter batch differences caused by reagent preparation errors, ensuring the reproducibility of clinical test results.

TOOS, as a representative of the new Trinder's reagent, redefines the accuracy and efficiency of adenosine deaminase detection through core advantages such as high sensitivity color development, strong anti-interference ability, and rapid reaction kinetics. With the advancement of molecular diagnostic technology, the potential application of TOOS in early screening of liver disease, diagnosis of tuberculosis infection, and immune function assessment will continue to be released, providing more reliable biochemical detection tools for clinical precision medicine.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. specializes in the research and production of TOOS and other chromogenic substrates. With rich production experience and professional technical and quality inspection personnel, we can provide excellent technical support and after-sales service. We have established cooperation with many domestic and foreign enterprises and received high praise. We have various online branches selling products, and offline we have large factories, professional technical teams, and advanced production equipment. We welcome users to visit!