Wuhan Desheng Biochemical Technology Co., Ltd

In biochemical and molecular biology experiments, the stability of buffering agents directly affects the reliability of experimental results. PIPES, as a commonly used biological buffer, has attracted much attention for its chemical stability. The stability of PIPES buffer is particularly outstanding at room temperature and neutral pH environment. In a solid state, it can be stored for several years in a dry place at room temperature after sealing without significant degradation. When in solution state, within its effective buffering range, it can be stabilized for several weeks at 4 ℃ refrigeration and maintained for 1-2 weeks at room temperature. The degradation products are minimal, mainly trace amounts of piperazine derivatives. This provides convenience for experimental operations and reduces the hassle of frequently preparing buffer solutions. The temperature has a significant impact on the stability of PIPES. When heated for a short period of time at 60-80 ℃, its structure is basically stable and can meet the needs of some mild heating experiments. But do not perform high-pressure sterilization, as high temperature and pressure can cause slight decomposition, producing by-products such as piperazine and sulfonic acid. This not only reduces buffering capacity, but may also release trace amounts of harmful substances that affect biological samples, such as cells and enzymes. In terms of lighting, PIPES is relatively stable to ordinary indoor lighting, but long-term exposure to strong light (such as ultraviolet light) may result in slow degradation due to photooxidation. Therefore, the solution needs to be stored in a brown bottle away from light to delay the degradation process. PIPES exhibits good compatibility in interactions with chemical substances. It is relatively stable against common oxidants and not easily oxidized; Not sensitive to reducing agents and can coexist with sulfur-containing reducing agents, suitable for experiments that require a reducing environment, such as protein purification. More importantly, its coordination ability with most metal ions (such as Na ⁺, K ⁺, Ca ² ⁺, Mg ² ⁺) is weak, making it difficult to form stable complexes. This characteristic gives it a significant advantage in reaction systems containing metal ions (such as enzyme reactions and cell culture), and it will not interfere with experiments due to chelation of metal ions. This is a major highlight compared to buffering agents such as BICINE and HEPES. There are three main degradation pathways for PIPES: under strong acidic (pH12) conditions, the bond between the sulfonic acid group and the ethane group may break, releasing sulfonic acid groups and piperazine derivatives; When the temperature exceeds 100 ℃, the piperazine ring may partially open, producing amine and carboxylic acid by-products; Under UV irradiation, it may cause oxidation of the piperazine ring, resulting in the formation of imines or hydroxyl derivatives. Although degradation products are usually not highly toxic, they can reduce buffering capacity and extreme conditions should be avoided. In practical applications, controlling stability is crucial. The solution is recommended to be prepared and used immediately. If storage is required, it can be adjusted to pH 6.5-7.0, stored at 4 ℃ in the dark, and avoid repeated freezing and thawing. If aseptic treatment is required, 0.22 μ m filter membrane can be used for filtration. Meanwhile, PIPES can coexist with most salts, low concentration organic solvents (such as ethanol, DMSO), and biological reagents (enzymes, proteins) without worrying about stability conflicts. In summary, PIPES exhibits excellent chemical stability under conventional experimental conditions and is widely used in biological systems. As long as the storage and usage conditions are reasonably controlled, its buffering performance can be fully utilized, providing reliable guarantees for biological experiments. Since the establishment of Desheng, we have always adhered to the core values of "service first". For product after-sales, we have an elite after-sales team who not only meticulously track and follow up on customer feedback information, but also provide professional product technical guidance. In addition, we highly value every suggestion and opinion from our customers and actively adopt them to continuously optimize our services. Therefore, if you are looking for high-quality biological buffering agents, Desheng is undoubtedly your trusted choice, and we promise to do our best to meet your expectations.

The 92nd China International Medical Equipment Fair (CMEF) in 2025 will grandly kick off on September 26-29 at the China International Import and Export Fair in Guangzhou. As one of the most influential events in the global medical and health technology field, this year's CMEF is expected to gather thousands of enterprises from home and abroad to comprehensively showcase cutting-edge product technology and system solutions. Hubei Xindesheng Material Technology Co., Ltd. will showcase its full range of products at booth C06 in Hall 20.1, exploring the new future of diagnostic reagents with you! New Desheng's core product matrix launches a full-scale attack 1. High end biological buffer series Xindesheng has a complete range of biological buffer products, covering dozens of commonly used and customized types such as TRIS, HEPES, MOPS, etc., with a daily production capacity of up to 5 tons. The product has high purity and stable process, and can be widely used in fields such as molecular diagnosis, immune detection, cell culture, etc. It maintains the pH value of the reaction system and provides a solid guarantee for the reliability of experimental results. 2. IVD Diagnostic Core Raw Material Series Chromogenic substrate: It has high water solubility, high sensitivity, color stability, and wide system adaptability, supporting multiple color source selections and detection scenarios, significantly improving detection sensitivity and stability. Luminescent reagents: including luminol, isoluminol, and various functional acridine ester products, with high luminescence efficiency and fast reaction speed, meeting the diverse needs of chemiluminescence immunoassay. Enzyme preparations: covering high value-added projects such as cardiovascular, infectious disease, and tumor marker detection, with characteristics of high activity and high purity. 3. Blood collection reagent series As one of the earliest domestic enterprises to produce a complete set of blood collection tube additives, Xindesheng provides stable quality and complete product categories of blood collection tube reagents with mature technology and rich experience, and can also provide deep customization services to fully meet customers' personalized needs. Xin Desheng: Deeply cultivated for 20 years, trustworthy Hubei Xindesheng Material Technology Co., Ltd. was established in 2005. It is a national high-tech enterprise specializing in the research, development, production, and sales of blood test pre-processing reagents, in vitro diagnostic reagent raw materials, and biological buffering agents. The company adheres to the concept of "quality first, customer-oriented" and can provide professional customized development and large-scale production services. At present, Desheng is actively preparing for the construction of the new Huanggang factory. As of now, the main structure of Warehouse 3 has been successfully capped, and the construction of the gutter and roof system of Production Building 1 is proceeding efficiently. The new factory is committed to building a domestic base for high-end biological buffering agents, and the upgrading of production capacity is just around the corner. This will further consolidate Xindesheng's supply chain advantages and market competitiveness in the field of IVD raw materials. In person interaction, enjoy multiple benefits and values During the exhibition, Xindesheng sincerely invites you to visit booth C06 in Hall 20.1, participate in on-site interaction, and experience more exclusive benefits and opportunities for in-depth communication: 1. We not only provide you with a comfortable and cozy resting area and a variety of drinks and tea breaks, but also offer face-to-face communication with technical and marketing teams to obtain the latest product information and technical solutions, bringing new ideas and cooperation opportunities for your current project. 2. By visiting the booth for interaction, you can receive exquisite and practical gifts for free (including product samples, customized canvas bags, multifunctional USB drives, etc.). Each gift is designed to provide convenience for your daily research and development and work. 3. On site participation and interaction can also give priority to understanding the latest production line planning and customer exclusive support of Xindesheng, obtain more market trend interpretation and product customization resources, help reduce costs and increase efficiency, and seize market opportunities. Meet in Guangzhou and discuss the future together From September 26th to 29th, Hubei Xindesheng is looking forward to your visit at the C06 booth in Hall 20.1 of the China Import and Export Fair Complex (Pazhou Complex) in Guangzhou! We look forward to face-to-face communication with you on industry trends, sharing innovative achievements, and working together to promote the localization process of diagnostic reagent raw materials, creating new opportunities for cooperation. We are waiting for you in Guangzhou. See you soon!

Fluttering banners, embarking on a new journey, fighting fiercely for a hundred days to see the true chapter In the summer of 2025, on the hot land of innovation and entrepreneurship in Huanggang, Hubei, a modern chemical plant representing a new height in the industry is rising at an astonishing speed. Since the foundation laying and departure on July 18th, the Huanggang new factory project of Hubei Xindesheng Material Technology Co., Ltd. has embarked on a construction journey of racing against time with the spirit of "time waits for no one, seize the day and night". In just two months, the project team has written an impressive report card with wisdom and sweat, perfectly interpreting the profound connotation of "Desheng efficiency" with tangible construction results, injecting a shot in the arm for local economic development. Warehouse sealing: Warehouse center takes shape first On September 14, 2025, the project construction once again reached a milestone moment - the main structure of Warehouse 3 successfully completed the topping out work! This advanced design and fully functional storage facility has completed the main construction first, which means that the construction of the new factory has achieved significant results in this stage. The topping out of the warehouse not only creates conditions for subsequent internal decoration and equipment installation, but also ensures the efficient operation of the future factory logistics and warehousing system. At the topping out ceremony, the project leader stated, "The rapid topping out of Warehouse 3 is a testament to our careful organization and scientific construction, and also a good start for the on-time delivery of the entire project. Multi line joint promotion: Comprehensive construction momentum is unstoppable At the same time, the construction of other key nodes in the new factory area is also progressing efficiently and synchronously, presenting a busy scene of multiple blooming points and simultaneous progress. The construction of the gutter and roof system in the 1 # production plant is in full swing, creating favorable conditions for the installation of internal equipment in the plant; The circulating water tank supporting the factory area has completed the base treatment and entered the plastering construction stage, and the bottom plate formwork construction of the base water tank and the initial rainwater tank has also been fully carried out. The synchronous construction of these supporting facilities ensures the coordinated operation of production and environmental protection systems in the future factory area, reflecting New Desheng's high emphasis on sustainable development. Strategic positioning: Building a domestic base for high-end biological buffering agents The Huanggang New Factory project is committed to building a domestic high-end biological buffer production base, focusing on breakthroughs in key preparation technologies for a series of high-end biological buffers such as TRIS, HEPES, MOPS, etc. By constructing multiple intelligent production lines, the project will achieve a significant increase in the production of high-end biological buffering agents after it is put into operation, and it is expected that the annual output value will achieve a leapfrog growth. The construction of this base will effectively break the technological and market monopoly of foreign manufacturers in the field of high-end biological buffering agents. Anchor new goals and embark on a new journey The rapid progress of Huanggang's new factory construction is not only an important milestone in the development history of Xindesheng, but also a microcosm of China's intelligent manufacturing speed. From a blueprint to the initial scale of the factory building, from the groundbreaking ceremony to the topping out of the warehouse, the early completion of every milestone embodies the wisdom and sweat of all builders, demonstrating the corporate spirit of "pioneering innovation and pursuing excellence" of Xindesheng people. With the continuous advancement of the project, it will undoubtedly inject strong impetus into the future development of Xindesheng and contribute important strength to the high-quality development of the local economy.

For a long time, some studies have assumed that Bicine cannot interact with metal ions. However, an increasing number of facts indicate that Bicine can form stable binary or ternary complexes with metal ions, exhibiting certain chelating properties. The molecular structure of Bicine buffer is similar to that of glycine. This structure reduces the hydrolysis ability of the amino end, enhances overall stability, and also creates conditions for its binding with metal ions. Research has shown that Bicine can chelate with heavy metal ions such as copper (Cu), cadmium (Cd), and lead (Pb). However, this chelation effect is relatively weak and does not significantly interfere with the normal function of metal ions in most biochemical reactions, especially enzymatic reactions. In enzymatic reactions, metal ions often act as cofactors or coenzymes in the catalytic process, and their activity is highly influenced by various factors, with pH being one of the key factors. Extreme pH values can alter the binding state between metal ions and enzymes, thereby affecting catalytic activity. Bicine maintains pH stability in the solution while having weak chelation effect on metal ions, making it an ideal choice for enzymatic reactions that require the retention of metal ion activity. For example, in the serum guanine enzyme assay, the presence of Bicine can provide a stable pH environment for enzymatic reactions, and due to its weak binding properties with metal ions, it will not interfere with the role of metal ions in the reaction, ensuring the accuracy of the measurement results. From an application perspective, this characteristic of Bicine enables it to be widely used in many fields. In protein crystallization solution, Bicine can maintain the pH stability of the system without affecting the necessary interactions between proteins and metal ions due to excessive chelation of metal ions, which is beneficial for the growth and stability of protein crystals. Bicine acts as a buffer when extracting enzymes from various plant species, ensuring a suitable pH environment and maintaining metal ions in a free state that can participate in the composition of enzyme active centers or promote enzyme activity. In polymerase chain reaction (PCR), Bicine buffer system provides stable acid-base conditions for the reaction, while not hindering the reaction due to strong chelation of metal ions (such as magnesium ions, activators of Taq DNA polymerase in PCR reaction). Overall, the weak binding properties of the biological buffer Bicine with metal ions have more advantages than disadvantages in most biochemical and molecular biology applications. It provides a stable and suitable environment for numerous biochemical reactions that require the participation of metal ions, ensuring the authenticity and reliability of experimental results. With the continuous deepening of research and the expansion of application fields, fully understanding and reasonably utilizing the characteristics of Bicine and metal ions will inject new vitality into the development of related fields. The Bicine buffer produced by Hubei Xindesheng Material Technology Co., Ltd. has a low chloride ion content and all indicators meet relevant standards. In addition to Bicine buffer, Desheng actively researches and develops dozens of biological buffers such as TRIS and Hepes commonly used in the market. If you are interested, please click on the Desheng official website to learn more details! Overall, the weak binding properties of the biological buffer Bicine with metal ions have more advantages than disadvantages in most biochemical and molecular biology applications. It provides a stable and suitable environment for numerous biochemical reactions that require the participation of metal ions, ensuring the authenticity and reliability of experimental results. With the continuous deepening of research and the expansion of application fields, fully understanding and reasonably utilizing the characteristics of Bicine and metal ions will inject new vitality into the development of related fields. The Bicine buffer produced by Hubei Xindesheng Material Technology Co., Ltd. has a low chloride ion content and all indicators meet relevant standards. In addition to Bicine buffer, Desheng actively researches and develops dozens of biological buffers such as TRIS and Hepes commonly used in the market. If you are interested, please click on the Desheng official website to learn more details!